Search results for: the-urinary-proteome

The Urinary Proteome

Author : Alex J. Rai
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Urine is one of the most easily accessible biological samples, and it provides a treasure trove of molecules essential for clinical diagnostics. In The Urinary Proteome: Methods and Protocols, expert researchers review briefly the classical urine tests that are performed in the clinical laboratory and then delve into the state of the art methods for proteomic analysis using urine specimens. Featuring the most recent advances in sample preparation, data analysis, and methods and applications, the book contains a multitude of detailed methods, including procedural details for the identification and characterization of urine biomarkers, which hold great potential for the diagnosis and treatment of many different disease conditions. Written in the highly successful Methods in Molecular BiologyTM series format, chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and systematic, The Urinary Proteome: Methods and Protocols is an ideal guide for scientific researchers, clinical laboratorians, clinical and translational scientists, and others interested in the vital study of proteomics and biomarker discovery.

Urine Proteomics in Kidney Disease Biomarker Discovery

Author : Youhe Gao
File Size : 62.87 MB
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This book systematically summarizes the ideas and technologies used in urine proteome analysis. It argues that change is the core of biomarker definition since the body uses its homeostatic mechanisms to correct changes in the blood. This means that urine is probably a better source of biomarkers than blood. A roadmap to the urinary biomarker era is proposed, and researchers are reminded of the potential opportunities and risks in their study design. Kidney diseases are emphasized as they produce the most significant changes in urine. This book tries to show researchers and graduate students, who are in or entering the field, "all things considered" rather than "the current affair".

Renal and Urinary Proteomics

Author : Visith Thongboonkerd
File Size : 44.61 MB
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Based on research and results from the international Human Kidney and Urine Proteome Project, this handbook and reference presents current methods and protocols for the analysis of human kidney and urine. The world's leading experts cover everything from basic techniques for routine analysis to advanced methods for difficult samples or analytes. Written with the practitioner in mind, all the methods and techniques are described with step-by-step protocols backed by much valuable practical advice taken from real life experiments.

A Systematic Review and Assessment of the Urinary Proteome in Patients with Intestinal Segments Transposed Into the Urinary Tract

Author : Ghulam Nabi
File Size : 33.86 MB
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Acta Physiologica Hungarica

Author :
File Size : 84.3 MB
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Mini Reviews in Medicinal Chemistry

Author :
File Size : 45.49 MB
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Folia Biologica

Author :
File Size : 37.49 MB
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Quantitative Proteome Analysis

Author : Kazuhiro Imai
File Size : 59.49 MB
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This book focuses on the advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and reproducibility. It also concentrates on the effective applications of these methods that resulted in many discoveries of the role of the proteins expressed in living cells and biological fluids. The first part of the book focuses on the description of advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and, especially, reproducibility. The second part of the book focuses on providing concise descriptions of the effective applications of these methods to demonstrate how they have resulted in many important discoveries of the roles of the proteins expressed in living cells.

Applications of Proteinchip Array based Proteomics in Molecular Epidemiology and Toxicology

Author : Christine Marie Hegedus
File Size : 83.88 MB
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2D PAGE Sample Preparation and Fractionation

Author : Anton Posch
File Size : 76.65 MB
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Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enable you to quickly learn how to perform the methods yourself. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.

Urinary Proteome Analysis for Detection of Diabetic Nephropathy in Patients with Diabetes Mellitus and Arterial Hypertension

Author :
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American Journal of Respiratory and Critical Care Medicine

Author :
File Size : 32.68 MB
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Proteomic Profiling of Saliva for the Detection of Cancer

Author : Prasanna Ramachandran
File Size : 88.7 MB
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Proteomics in Nephrology

Author : Visith Thongboonkerd
File Size : 42.48 MB
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Since the publication of the first volume on proteomics in nephrology, methodologies and protocols for renal and urinary proteome analyses have been continuously improved, resulting in considerable progress towards clinical application. Proteomics not only contributes to a better understanding of the renal physiology and pathogenic mechanisms of kidney diseases, but also assists in the search for novel biomarkers for diagnostics and prognostics and supports the definition and development of new therapeutic targets and drugs for better therapeutic outcome. While the first volume focused mainly on an overview, technologies and methodologies, this volume highlights successful applications of proteomics to several kidney diseases, including acute kidney injury, nephrotic syndrome, diabetic nephropathy, renal allograft rejection, renal cell carcinoma, obstructive nephropathy, kidney stone disease, uremia, and others. Written by acclaimed experts in proteomics and nephrology, this book is an excellent resource of references for nephrologists, clinicians, pharmacists, other healthcare professionals, proteomists, physiologists, scientists, and trainees.

Neuroproteomics

Author : Andrew K. Ottens
File Size : 34.87 MB
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With the development of polypeptide ionization processes for mass spectrometry and the rapid processing of large-scale datasets with bioinformatics, researchers now have the tools necessary to propel neuroproteomics research to new levels. In Neuroproteomics: Methods and Protocols, experts in the neurological and analytical sciences present experimental details for applying proteomics to the study of the central nervous system (CNS) and its dysfunction through trauma and disease. Divided into four convenient sections, this collection covers CNS animal models used for neuroproteomics research, methods for separating and analyzing discrete subcomponents of the neuroproteome, large-scale approaches for CNS proteome characterization and quantification, as well as methods that evaluate biofluids and translate neuroproteomic results into clinical platforms. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neuroproteomics: Methods and Protocols includes the clear "how-to" information needed to critically assess what can be accomplished in the field and how to best perform and evaluate neuroproteomic experiments in one’s own research.

The First in Vivo Human Methionine Sulfide Proteome and the Impact of Smoking

Author : Abdulla Mohammed Abdulrahm Qassab
File Size : 74.36 MB
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Reactive oxygen species are naturally generated within the human body and they are known to modulate signaling pathway and mediate other physiological activities. However, excessive generation of ROS and the inability of body defense system in detoxifying them results in the so called "oxidative stress". Methionine has powerful antioxidant properties due to the presence of electronegative sulfur in its structure. Therefore, Met is readily oxidized, and methionine sulfoxide has been linked to several pathological conditions. The urinary proteome is an attractive candidate for the discovery of biomarkers to diagnose and classify health conditions because of the non-invasive collection procedure. However, protocols developed for the analysis of the urinary proteome have limitations both in identification of proteins and eliminating false positives. Urine samples were collected from smoker and non-smokers participants. Proteins were concentrated and concentrates then were subjected to separation via SDS-PAGE gel electrophoresis technique. Gel was cut out into five sections including the band representing intact HSA. Gel sections then were digested with trypsin. Total proteins and levels of methionine sulfoxide in the resulting peptides were assessed by mass spectrometry. Data analysis was performed using Scaffold and Skyline software packages. Correspondence with predicted retention times, MS/MS sequence information, and a minimum of two tryptic peptides were required to reduce false positives. Proteins were matched to 1023 UniProtKB identification numbers, 467 of which have not been previously reported in the human urinary proteome. Analysis of methionine sulfoxide proteome revealed 392 peptides mapped to 198 proteins that were detected with a significant fold change in smokers. Regions of gels with proteins of lower mass showed significant higher levels of oxidation in smokers compared to control, suggesting an association between oxidative caused by smoking and fragmentation of proteins.

Clinical Proteomics

Author : Jennifer E. Van Eyk
File Size : 25.53 MB
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Unparalleled in its scope and depth, this book brings together proteomic approaches in diagnosis and treatment from all clinical fields, including clinical toxicology. The result is a new discipline in molecular medicine that will revolutionize the treatment and prevention of cancer, stroke and other severe diseases. Following an overview of clinical proteomics, the authors look at the technologies available, before moving on to cancer, cardiopulmonary disease, diabetes and stroke. A whole section is devoted to toxicity and the work is rounded off with a discussion of the future of clinical proteomics.

Brenner Rector s the Kidney

Author : Barry M. Brenner
File Size : 79.32 MB
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Clinical Science

Author :
File Size : 55.70 MB
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The Urine Proteome of Kidney Transplant Patients

Author : Arne Füldner
File Size : 88.75 MB
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