Search results for: spermatogenesis

Proteomics of Spermatogenesis

Author : G. S. Gupta
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Recent developments of experimental techniques in cellular and molecular biology have made it possible to understand the molecular biology of male gametogenesis in greater detail. This book focuses on the description of specialized proteins, which are dominantly and/or specifically expressed in germ cells and localized in spermatozoa. There is an urgent need to classify proteins of spermatogenic cells with a view of their functions, and their applications in the regulation of fertility and in understanding infertility. The understanding of structural properties of male germ cell specific proteins can offer vulnerable points for targeted intervention in testis without generalized effects on stages of spermatogenesis. Besides targeted action in male germ cells, sperm specific proteins and polypeptides may also offer potential application in the development of a contraceptive vaccine.

Biology of Spermatogenesis and Spermatozoa in Mammals

Author : Sardul S. Guraya
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Molecular Mechanisms in Spermatogenesis

Author : C.Y. Cheng
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In the past thirty years, significant advances have been made in the field of reproductive biology in unlocking the molecular and biochemical events that regulate spermatogenesis in the mammalian testis. It was possible because of the unprecedented breakthroughs in molecular biology, cell biology, immunology, and biochemistry. In this book entitled, Molecular Mechanisms in Spermatogenesis, a collection of chapters has been included written by colleagues on the latest development in the field using genomic and proteomic approaches to study spermatogenesis, as well as different mechanisms and/or molecules including environmental toxicants and transcription factors that regulate and/or affect spermatogenesis. The book begins with a chapter that provides the basic concept of cellular regulation of spermatogenesis. A few chapters are also dedicated to some of the latest findings on the Sertoli cell cytoskeleton and other molecules (e.g., proteases, adhesion proteins) that regulate spermatogenesis. These chapters contain thought-provoking discussions and concepts which shall be welcomed by investigators in the field. It is obvious that many of these concepts will be updated and some may be amended in the years to come. However, they will serve as a guide and the basis for investigation by scientists in the field.

Sertoli Cell Metabolism and Spermatogenesis

Author : Pedro F. Oliveira
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This book is focused on Sertoli cell physiology and its role in the spermatogenic event. These cells, known as “nurse cells”, are essential for the normal development of germ cells by offering not only physical support and creating an immune-privileged environment, but also for providing nutritional support. The presence of Sertoli cells promotes the establishment of the appropriate microenvironment so that spermatogenesis may occur. Spermatogenesis maintenance in vivo is highly dependent on the metabolic cooperation established between Sertoli cells and developing germ cells. For many years this metabolic cooperation between testicular cells has been disregarded, but recent advances have highlighted the relevance of these processes for male fertility. Thus, the understanding of the functioning and regulation of these metabolic processes is a crucial step to identify key mechanisms associated with Sertoli cell (dys)function and to enlighten their influence on male fertility.

Spermatogenesis Fertilization Contraception

Author : Eberhard Nieschlag
File Size : 63.8 MB
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"Proceedings of the Schering Foundation Workshop on 'Basic Mechanisms of Reproduction and Male Fertility Control' ... held in Berlin on October 31 to November 2, 1991"--Pref.

Spermatogenesis

Author : Lori Barnard
File Size : 39.36 MB
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Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.

Spermatogenesis in Boars

Author : James Joseph Kennelly
File Size : 75.99 MB
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Illustrated Pathology of Human Spermatogenesis

Author : Adolf Friedrich Holstein
File Size : 79.86 MB
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Molecular Aspects of Spermatogenesis

Author : Dominic Poccia
File Size : 35.47 MB
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Spermatogenesis and Oogenesis in Musca Domestica L

Author : Allen Lee French
File Size : 23.76 MB
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The Process of Spermatogenesis in Animals

Author : Edward C. Roosen-Runge
File Size : 74.15 MB
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An Ultrastructural Study of Spermatogenesis in Two Species of Rana

Author : Gary Raymond Poirier
File Size : 22.15 MB
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Electronmicroscopy and Cytochemistry of Spermatogenesis and Fertilization in the Rat and Hamster

Author : Daniel Gabriel Szollosi
File Size : 23.53 MB
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Gamma Irradiation Effects on Spermatogenesis and Reproductive Potential of Navel Orangeworm Paramyelois Transitella Walker

Author : Usha Kiran Gogia
File Size : 26.7 MB
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SPERMATOGENESIS IN THE BELOSTOMATIDAE THE CHROMOSOMES AND CYTOPLASMIC INCLUSIONS IN THE MALE GERM CELLS OF BELOSTOMA FLUMINEUM SAY LETHOCERUS AMERICANUS LEIDY AND BENACUS GRISEUS SAY

Author : Arthur Merton Chickering
File Size : 36.78 MB
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Reproductive Biology of Invertebrates

Author : K. G. Adiyodi
File Size : 22.4 MB
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Spermatogenesis in the Sea Star Patiria Miniata Brandt 1835

Author : Karen Kay Davis
File Size : 55.99 MB
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The Role of Testicular Hyaluronidase in Spermatogenesis and Fertilization

Author : Jinshin Yamane
File Size : 37.33 MB
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Some Factors Affecting Spermatogenesis and Fertility of Dairy Bulls

Author : Robert Charles Lewis
File Size : 44.77 MB
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Bovine Spermatogenesis and Production of Spermatids from Transplanted Testis Tissue

Author : Michael Thomas Kaproth
File Size : 74.68 MB
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We developed three bovine spermatogenesis models, maintaining cell-cell associations and somatic cell support for spermatid production from prepubertal testis tissue: testis-explant transplantation, testis-tissue culture and spermatogonial transplantation. Detailed labeled images were made of normal bovine spermatogenesis and testis tissue cytology based on classical studies. Spermatids were produced in prepubertal bovine testis tissue explants transplanted to adult intact nude mice subcutaneous flanks. Increased germ cell progression and graft morphology characteristics were determined. Flank, but not intratesticular transfers produced spermatids. Longer transplantation-recovery intervals increased germ cell progression. Spermatids were found in 42.3% of 20--26 week grafts. Cultured and fresh explants were equally successful. Recipient age and testosterone supplementation did not affect germ cell progression. Tubule diameter, cell numbers and Leydig cell development was associated with increased germ cell progression. Spermatid function was determined with ICSI and embryo culture. Spermatids from 11, 15, 20 week grafts injected into oocytes, generated 97 zygotes, 72 3-day embryos, and 44 10-day blastocysts. Highest-graded blastocysts arose from 15 week grafts. DNA of 6 blastocysts was Y-chromosome positive, confirming biparental origin and spermatid developmental competence. Prepubertal testis tissue was cultured through 3 months. Aggregates and floating rafts of heterogeneous cells produced spermatogonial mitosis and early spermatocytes. Late spermatocytes and spermatids were tentatively detected but not positively confirmed. Spermatogonial transplantation was performed in untreated and busulfan-treated mice rete testis and efferent duct injection. In untreated mice, rete injection was successful. In busulfan-treated immune-deficient mice, rete injection was rarely successful due to poor testis tone. Efferent duct injections were successful but limited by tedious injection-site epididymal fat dissection. Injected bovine spermatogonia did not produce spermatids with distinctive bovine head morphology. Culture conditions were assessed for ability to maintain prolonged testis tissue functionality. Testicular cell conversion of testosterone to estradiol was maximal at 28--54 days. Culture beyond 71 days, but neither donor maturity nor tissue dissociation, significantly affected estradiol production. This allows for future transfection and screening treatments towards potential directed germ-line modification. These results demonstrate developmentally competent spermatids can be produced from bovine testis tissue grafts supporting the potential of this approach for producing and genetically modifying haploid germ cells.