Search results for: micropropagation

High Tech and Micropropagation V

Author : Y.P.S. Bajaj
File Size : 67.93 MB
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This volume, fifth in the series High-Tech and Micropropagation, contains 24 chapters arranged in the following three sections: I. Vegetables and Fruits: garlic, Amaranthus, Brassica oleracea, pepper, watermelon, cassava, banana, Myrtus communis, passionfruit, Polymnia sonchifolia, pepino, and spinach. II. Grasses: bamboos, Caustis dioica, Dendrocalamus, Miscanthus x giganteus, sugarcane. III. Trees: Aegle marmelos, Eucalyptus, Fraxinus excelsior, Juglans cinerea, Pinus virginiana, Prosopis, and Ulmus. This book is of use to research workers, advanced students, and teachers in the fields of horticulture, forestry, botany, and plant biotechnology in general, and also to individuals interested in industrial micropropagation.

Protocols for Micropropagation of Woody Trees and Fruits

Author : S.Mohan Jain
File Size : 67.21 MB
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Micropropagation has become a reliable and routine approach for large-scale rapid plant multiplication, which is based on plant cell, tissue and organ culture on well defined tissue culture media under aseptic conditions. A lot of research efforts are being made to develop and refine micropropagation methods and culture media for large-scale plant multiplication of several number of plant species. However, many forest and fruit tree species still remain recalcitrant to in vitro culture and require highly specific culture conditions for plant growth and development. The recent challenges on plant cell cycle regulation and the presented potential molecular mechanisms of recalcitrance are providing excellent background for understanding on totipotency and what is more development of micropropagation protocols. For large-scale in vitro plant production the important attributes are the quality, cost effectiveness, maintenance of genetic fidelity, and long-term storage. The need for appropriate in vitro plant regeneration methods for woody plants, including both forest and fruit trees, is still overwhelming in order to overcome problems facing micropropagation such as somaclonal variation, recalcitrant rooting, hyperhydricity, polyphenols, loss of material during hardening and quality of plant material. Moreover, micropropagation may be utilized, in basic research, in production of virus-free planting material, cryopreservation of endangered and elite woody species, applications in tree breeding and reforestation.

High Tech and Micropropagation VI

Author : Y.P.S Bajaj
File Size : 24.3 MB
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This volume, fifth in the series High-Tech and Micropropagation, contains 24 chapters arranged in the following three sections: I. Vegetables and Fruits: garlic, Amaranthus, Brassica oleracea, pepper, watermelon, cassava, banana, Myrtus communis, passionfruit, Polymnia sonchifolia, pepino, and spinach. II. Grasses: bamboos, Caustis dioica, Dendrocalamus, Miscanthus x giganteus, sugarcane. III. Trees: Aegle marmelos, Eucalyptus, Fraxinus excelsior, Juglans cinerea, Pinus virginiana, Prosopis, and Ulmus. This book is of use to research workers, advanced students, and teachers in the fields of horticulture, forestry, botany, and plant biotechnology in general, and also to individuals interested in industrial micropropagation.

Pathogen and Microbial Contamination Management in Micropropagation

Author : Alan C. Cassells
File Size : 77.82 MB
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This book is based mainly on invited and offered papers presented at the Second International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures held at University College, Cork, Ireland in September 1996, with additional invited papers. The First International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures was held at the same venue in 1987 and was published as Acta Horticulturae volume 225, 1988. In the intervening years there have been considerable advances in both plant disease diagnostics and in the development of structured approaches to the management of disease and microbial contamination in micropropagation. These approaches have centred on attempts to separate, spatially, the problems of disease transmission and laboratory contamination. Disease-control is best achieved by establishing pathogen-free cultures while laboratory contamination is based on subsequent good working practice. Control of losses due to pathogens and microbial contamination in vitro addresses, arguably, the most importance causes of losses in the industry; nevertheless, losses at and post establishment can also be considerable due to poor quality microplants or micro-shoots. In this symposium, a holistic approach to pathogen and microbial contamination control is evident with the recognition that micropropagators must address pathogen and microbial contamination in vitro, and diseases and microplant failure at establishment. There is increasing interest in establishing beneficial bacterial and mycorrhizal association with microplants in vitro and in vivo.

Micropropagation of Woody Plants

Author : M.R. Ahuja
File Size : 44.64 MB
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This volume covers recent advances in the vegetative propagation of woody plants by tissue culture. A wide range of topics relevant to micropropagation of woody plants are discussed by renowned international scientists. These include cellular contro of morphogenesis, light regimes in tissue culture, maturation and rejuvenation, synthetic seed, genetics of micropropagated plants, haploid embryogenesis, protoplast culture, and acclimatization of ex vitro woody plants. In addition to micropropagation of selected woody plants, both gymnosperms and angiosperms, this volume also includes in vitro genetic selection, strategic planning for application of biotechnology for genetics and breeding, and clonal options for woody plant improvement. A balanced view of both perspectives and limitations of woody plant micropropagation is presented.

Technical Guideline on Seed Potato Micropropagation and Multiplication

Author : Raymond A. T. George
File Size : 47.10 MB
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Transfer from in vitro to compost for tuber production; Field multiplication of tubers derived from cuttings; Disease indexing; Health and safety; Composition of medium used for meristem culture; Composition of medium used for cutting culture; Testing for bacterial ring rot.

Micropropagation

Author : P. Debergh
File Size : 78.75 MB
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Micropropagation is a technology that has developed within the past 30 years. Earlier overviews of plant tissue culture have reviewed micropropagation as just one of many tissue culture procedures in use. Since the applications of this technology have multiplied so rapidly in recent years, we decided that a specific overview of the technology was now appropriate Our book begins with a review of the general principles of tissue culture as applied to micropropagation. This review is concise since the general topic has been covered in numerous other books and reviews. The basic principles of laboratory design and construction are summarized in the second chapter. Common problems encountered in micropropagation, both during and after culture, are examined in detail in four chapters. As micropropagation developed from a laboratory curiosity to a commercial industry, different considerations became important. These are discussed in two chapters. An attempt has been made to assess the current status of commercial production around the world. This has been difficult because commercial production figures are often closely guarded and little has been done to collect statistics on this growing industry. Applications to a broad range of crops are discussed in a series of chapters. These try to report the state of the art in each area, but since applications for some crops are much more advanced than for others, the focus of these chapters varies depending upon the progress that has been made.

Photoautotrophic sugar free medium Micropropagation as a New Micropropagation and Transplant Production System

Author : Toyoki Kozai
File Size : 78.30 MB
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This book provides two basic concepts on plant propagation and value-added transplant production in a closed structure with artificial lighting: 1) photoautotrophic (sugar-free medium, photosynthetic or inorganic nutrition) micropropagation systems, and 2) closed transplant production systems with minimum resource consumption and environmental pollution. This book also describes the methodology, technology and practical techniques employed in both systems, which have been commercialized recently in some Asian countries such as China and Japan. We often use a closed structure such as a tissue culture vessel, a culture room, a growth chamber, a plant factory with lamps, and a greenhouse to propagate plants and produce transplants. Main reasons why we use such a closed structure is: 1) higher controllability of the environment for desired plant growth, 2) easier protection of plants from damage by harsh physical environment, pathogens, insects, animals, etc, 3) easier reduction in resource consumption for environmental control and protection, and 4) higher quality and productivity of plants at a lower cost, compared with the plant propagation and transplant production under rain, wind and sunlight shelters and in the open fields. Thus, there should be some knowledge, discipline, methodology, technology and problems to be solved on plant propagation and transplant production common to those closed structures, regardless of the types and sizes of the closed structure.

High Tech and Micropropagation III

Author : Y. P. S. Bajaj
File Size : 32.54 MB
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Presenting the state of the art of tissue culture and in vitro propagation of vegetable and tuber crops, medicinal and aromatic plants, fibre and oilseed crops, and grasses, this book complements the previous two volumes on High-Tech and Micropropagation, which concentrated on special techniques (Vol.17) and trees and bushes of commercial value (Vol.18). The specific plants covered here include asparagus, lettuce, horse radish, cucumber, potato, cassava, sweet potato, artichoke, yams, cardamom, fennel, celery, thyme, leek, mentha, turmeric, lavender, agave, yucca, cotton, jute, sunflower, ryegrass, zoysiagrass, and various species of Aconitum, Artemisia, Camelia, Centaurium, Digitalis, Dioscorea, Glehnia, Levisticum, Parthenium, and Pinella. The book is of use to advanced students, teachers and research workers in the field of pharmacy, horticulture, plant breeding and plant biotechnology in general, and also to individuals interested in industrial micropropagation.

High Tech and Micropropagation I

Author : Y. P. S. Bajaj
File Size : 84.12 MB
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Presented here is another classic from this series and deals with general aspects of micropropagation of plants for commercial exploitation. It includes chapters on setting up a commercial laboratory, meristem culture, somatic embryogenesis, factors affecting micropropagation, disposable vessels, vitrification, acclimatization, induction of rooting, artificial substrates, cryopreservation and artificial seed. Special emphasis is given on modern approaches and developing technologies such as automation and bioreactors, robots in transplanting, artificial intelligence, information management and computerized greenhouses for en masse commercial production of plants.