Search results for: genetic-engineering-of-plants

Genetic Engineering of Plants

Author : Tsune Kosuge
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William C. Taylor Department of Genetics University of California Berkeley, California 94720 It is evident by now that there is a great deal of interest in exploiting the new technologies to genetically engineer new forms of plants. A purpose of this meeting is to assess the possibilities. The papers that follow are concerned with the analysis of single genes or small gene families. We will read about genes found within the nucleus, plastids, and bacteria which are responsible for agri culturally important traits. Given that these genes can be isolated by recombinant DNA techniques, there are two possible strategies for plant engineering. One involves isolating a gene from a cultivated plant, changing it in a specific way and then inserting it back into the same plant where it produces an altered gene product. An example might be changing the amino acid composition of a seed pro tein so as to make the seed a more efficient food source. A second strategy is to isolate a gene from one species and transfer it to another species where it produces a desirable feature. An example might be the transfer of a gene which encodes a more efficient pho tosynthetic enzyme from a wild relative into a cultivated species. There are three technical hurdles which must be overcome for either strategy to work. The gene of interest must be physically isolated.

Plant Genetic Engineering

Author : John H. Dodds
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This book was first published in 1985. For those working in molecular biology, this book describes techniques in plant genetic research and the practical application of genetic engineering to important crop plants such as the potato. The various chapters detail methods used for the genetic modification of plants, including protoplast fusion and the use of Agrobacterium and viruses as vectors for plant genes. The types of agricultural and industrial processes that will be improved by these technologies are indicated throughout the book. The contributors to this volume have prepared a comprehensive and pertinent bibliography that is a key to the literature. Their scientific reports will enlighten advanced students, research workers and technicians in botany, biochemistry and biotechnology. All scientists in plant molecular biology, genetics, biochemistry and agriculture should find this book a valuable aid in their understanding of current techniques, principles and applications in plant genetic engineering.

Plant Genetic Engineering

Author : A.D. Arencibia
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Plant biotechnology offers important opportunities for agriculture, horticulture, and the pharmaceutical and food industry by generating transgenic varieties with altered properties. This is likely to change farming practice and reduce the potential negative impact of plant production on the environment. This volume shows the worldwide advances and potential benefits of plant genetic engineering focusing on the third millennium. The authors discuss the production of transgenic plants resistant to biotic and abiotic stress, the improvement of plant qualities, the use of transgenic plants as bioreactors, and the use of plant genomics for genetic improvement and gene cloning. Unique to this book is the integrative point of view taken between plant genetic engineering and socioeconomic and environmental issues. Considerations of regulatory processes to release genetically modified plants, as well as the public acceptance of the transgenic plants are also discussed. This book will be welcomed by biotechnologists, researchers and students alike working in the biological sciences. It should also prove useful to everyone dedicated to the study of the socioeconomic and environmental impact of the new technologies, while providing recent scientific information on the progress and perspectives of the production of genetically modified plants. The work is dedicated to Professor Marc van Montagu.

Genetic Engineering of Crop Plants

Author : G. W. Lycett
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Genetic Engineering of Crop Plants is a proceeding of The 49th Nottingham Easter School in Agricultural Science, which was held at Sutton Bonington on April 17-21, 1989. This symposium discussed progress in the generation of crop species resistant to herbicides, viruses, and insects. The book discusses topics such as the genetic manipulation in plants; genetic engineering of crops for insect and herbicide resistance; the expression of heat shock gene in transgenic plants; and tuber-specific gene expression. The book also covers topics such as regulation of gene expression in transgenic tomato plants; the molecular biology of pea seed development; and the regulatory elements of maize storage protein genes. The text is recommended for experts in the field of botany, agriculture, and genetics who would like to know more about the improvement of crop plants through genetics.

Genetic Modification of Plants

Author : Frank Kempken
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Conceived with the aim of sorting fact from fiction over genetically modified (GM) crops, this book brings together the knowledge of 30 specialists in the field of transgenic plants. It covers the generation and detection of these plants as well as the genetic traits conferred on transgenic plants. In addition, the book looks at a wide variety of crops, ornamental plants and tree species that are subject to genetic modifications, assessing the risks involved in genetic modification as well as the potential economic benefits of the technology in specific cases. The book’s structure, with fully cross-referenced chapters, gives readers a quick access to specific topics, whether that is comprehensive data on particular species of ornamentals, or coverage of the socioeconomic implications of GM technology. With an increasing demand for bioenergy, and the necessary higher yields relying on wider genetic variation, this book supplies all the technical details required to move forward to a new era in agriculture.

Plant Protoplasts and Genetic Engineering II

Author : Y. P. S. Bajaj
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Genetic engineering through DNA recombinants and the in vitro manipulation of isolated protoplasts has recently attracted much atten tion in agricultural biotechnology, and has greatly advanced during the last 5 years. In an earlier book, Plant Protoplasts and Genetic Engineer ing I, methods for the isolation, fusion and culture of protoplasts were reviewed and the regeneration of complete plants from isolated pro toplasts of rice, potato, soybean, linseed, cabbage, chicory, lettuce, but terbur, orchids, citrus and some other tree species, and interspecific and intergeneric somatic hybrids in Lycopersicon, Petunia, Nicotiana, Solanum, Glycine, Citrus, Brassica, Medicago and Trifolium spp. were discussed. The present volume, Plant Protoplasts and Genetic Engineering II, deals with some of the newer techniques such as microinjections, elec trofusion, flow cytometry, uptake and integration of DNA, nuclei, iso lated chromosomes by plant protoplasts and the subsequent regeneration of transgenic plants. The literature on the DNA recombinants and genetic transformation, both Agrobacterium-mediated and direct gene transfer in agricultural crops and trees, such as poplars, is reviewed, and the uses of cytoplasts and miniprotoplasts in genetic manipulation are highlighted.

Transgenic Plants in Agriculture

Author : Axel Kahn
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Axel Kahn's book, published late in 1996, which provided an overview of the opinions expressed by the Commission of Biomolecular Engineering about genetically modified plants, was a great success. Given the scale and importance of the phenomenon, the French Ministry of Agriculture and publishers John Libbey Eurotext have decided to publish an English-language version of this fundamental book about the introduction and development of genetically modified plants. For some years now, plant biotechnology, especially genetic engineering, has enabled us to modify the cycle of plant production, strengthening resistance to weedkillers and pests, improving yields and quality, adapting plants to unfavourable environments and creating new species. In France, the Biomolecular Engineering Commission (CGB) is responsible for authorising the marketing of these modified products. Over the past ten years it has certified 450 new products for public consumption. This book, which is suitable for the general public, reports on the experience acquired by the CGB and the studies it has conducted: What are the potential risks associated with so-called transgenetic plants? Are there any undetectable phenomena involved? - How can such plants be produced more safely? Axel Kahn is a world-renowned geneticist and clinician, chaired the Biomolecular Engineering Commission until 1998. Here he explains the "philosophy" of the CGB, which has gained unrivalled experience in Europe, and sets out ethical and scientific guidelines for the use of genetic engineering techniques.

Genetic Engin of Plants for Crop Improvement

Author : Rup Lal
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Genetic Engineering of Plants for Crop Improvement discusses current genetic engineering methods for plants and addresses the commercial opportunities for transgenic plants. Topics covered include Agrobacterium-mediated transformations, the use of electroporation, PEG-mediated transformation, microinjection, the microprojectile bombardment method, and the electrical discharge particle acceleration method. A concise account of the resistance of transgenic plants to insect attack, viral infection, and herbicides has also been provided. Possibilities for genetic manipulation for proteins that have superior nutritional properties are discussed, and a brief account of tests confirming the safety and commercial validity of transgenic plants is included. Genetic Engineering of Plants for Crop Improvement provides valuable information for researchers and students in plant biotechnology, plant gene manipulation, molecular biology, and all areas of the life sciences.

Introduction To Genetic Engineering Of Crop Plants Aims And Achievements

Author : A. Rashid
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Transgene technology since its inception, about two decades ago, has progressed rapidly providing platform for discovery, product design and novel plants which are improved source of food, feed, chemicals and drugs. This knowledge is changing rapidly by which plants develop their architecture to survive, abiotic and biotic stress, and become resistant to herbicides, pests and pathogens. Also the scene is set for a change from traditional farming to molecular farming. Moreover, gene silencing from a bane has turned out to be a boon, opening new vistas in genetic engineering of crop plants. In this book one can find an up-to-date account of aims and achievements of genetic engineering of crop plants. This book will useful for the undergraduate students of Botany, Biotechnology and Agriculture.

Plant Protoplasts and Genetic Engineering I

Author : Y. P. S. Bajaj
File Size : 23.95 MB
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Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-· toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean prepara tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Inter national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, in corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite.