Search results for: gene-amplification-in-mammalian-cells

Gene Amplification in Mammalian Cells

Author : Rodney E. Kellems
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Serves as a comprehensive review to the substantial impact of gene amplification in molecular biology, genetic engineering and medical science. The book covers the mechanism of gene amplification, organization and structure of amplified genes.

Gene Transfer and Expression in Mammalian Cells

Author : S.C. Makrides
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The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors. This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.

Mammalian Cell Biotechnology in Protein Production

Author : Hansjörg Hauser
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„Hauser und Wagner haben die neuen Möglichkeiten der Mammalian Cell Biology sehr anregend dargestellt.“ Prof. Dr. Hans Fritz, Ludwig-Maximilians-Universität München

Animal Cell Biotechnology

Author : Hansjörg Hauser
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This book introduces fundamental principles and practical application of techniques used in the scalable production of biopharmaceuticals with animal cell cultures. A broad spectrum of subjects relevant to biologics production and manufacturing are reviewed, including the generation of robust cell lines, a survey of functional genomics for a better understanding of cell lines and processes, as well as advances in regulatory compliant upstream and downstream development. The book is an essential reference for all those interested in translational animal cell-based pharmaceutical biotechnology.

Dictionary of DNA and Genome Technology

Author : Paul Singleton
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DNA technology is evolving rapidly, with new methods and a fast-growing vocabulary. This unique dictionary offers current, detailed and accessible information on DNA technology to lecturers, researchers and students throughout the biomedical and related sciences. The third edition is a major update, with over 3000 references from mainstream journals and data from the very latest research – going well beyond the remit of most science dictionaries. It provides clear explanations of terms, techniques, and tests, including commercial systems, with detailed coverage of many important procedures and methods, and includes essay-style entries on many major topics to assist newcomers to the field. It covers topics relevant to medicine (diagnosis, genetic disorders, gene therapy); veterinary science; biotechnology; biochemistry; pharmaceutical science/drug development; molecular biology; microbiology; epidemiology; genomics; environmental science; plant science/agriculture; taxonomy; and forensic science.

Mechanisms of Carcinogenesis

Author : Elizabeth K. Weisburger
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but also the possibility of intervention in specific stages. In Human behavior, including stress and other factors, plays an important role in neoplasia, although too little is known addition, variables which affect cancer development as well on the reasons for such development. Carcinogens, which as some endogenous factors can be better delineated help initiate the neoplastic process, may be either synthetic through such investigations. The topics of this volume encompass premalignant non or naturally-occurring. Cancer causation may be ascribed to invasive lesions, species-specific aspects of carcinogenicity, certain chemicals, physical agents, radioactive materials, viruses, parasites, the genetic make-up of the organism, and radiation, viruses, a quantum theory of carinogenesis, onco bacteria. Humans, eumetazoan animals and vascular plants genes, and selected environmental carcinogens. are susceptible to the first six groups of cancer causes, whe reas the last group, bacteria, seems to affect only vascular plants. Neoplastic development may begin with impairment ofJmdy defenses by a toxic material (carcinogen) which acts as an initiator, followed by promotion and progression to an overt neoplastic state. Investigation of these processes Series Editor Volume Editor allows not only a better insight into the mechanism of action Hans E. Kaiser Elizabeth K. Weisburger vii ACKNOWLEDGEMENT Inspiration and encouragement for this wide ranging project on cancer distribution and dissemination from a comparative biological and clinical point of view, was given by my late friend E. H. Krokowski.

The Science of Cancer Treatment

Author : B.A. Ponder
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Many of the most effective treatments for disease have been discovered empir ically. Nowadays, however, we think that understanding the biology of a disease will lead us to design better treatments, and to improve the application of treatments we already have. To accomplish this, vast sums are expended on cancer research. Even so, to the casual observer of clinical oncology the proliferation of studies and trials of ever-different combinations of therapies looks like empiricism, at the best. In the first part of this book, we have asked practising clinicians in different specialities to assess the contributions of biology and of empiricism to current approaches to treatment. In the second part, we have asked researchers in different areas of biology applied to cancer to assess the present and likely future impact of their type of biology on cancer treatment and control. IX 1 Surgery M.BAUM OBJECTIVES OF CANCER TREATMENT The objectives of cancer treatment can be defined according to population requirements or according to the needs of the individual. As far as the population is concerned, Government authorities are entitled to expect that cancer treatment will lead to mortality reductions and cost containment.

Journal of Bioscience and Bioengineering

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Gene and Chromosome Analysis

Author : Kenneth W. Adolph
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Gene and Chromosome Analysis, Part C is a practical laboratory guide to current techniques of molecular biology and genetics. The focus of the volume is on eukaryotic cells, particularly human cells and other eukaryotic model systems. * Methods presented for ease of use and ready adaptation to new systems. * Detailed experimental protocols included for: * Gene analysis * Transcription analysis * Transcription factors * Splicing * Differential display method * PCR * Recombinant proteins * Antibody methods * Receptors * Gene amplification strategies * Chromosone analysis * Yeast artificial chromosomes (YACs) * Microcloned DNA markers * Somatic cell genetic methods * In sity hybridization * Chromosomal proteins and DNA * Chromatin reconstitution * Telomerase and telomeres * Nucleoprotins * DNA damage * Disease and development * Carcinogen analysis * Plasmid DNA transfer * Mouse model

Mechanisms of Cancer Metastasis

Author : Kenneth V. Honn
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The past twenty years have witnessed significant advances in the treatment of cancer by surgery and radiation therapy. Gains with cytotoxic chemotherapy have been much more modest. Of the approximately 900,000 newly diagnosed cases of cancer each year, 50010 result in death of the patient. The primary cause of these deaths is metastasis. Although the term metastasis was first coined by Recamier in 1829, only in the past ten years have there been intensive scientific investigations into the mechanisms by which tumor cells metastasize. What has emerged is a complex process of host-tumor cell interactions which has been termed the metastatic cascade. Due to the complexity of the metastatic process, the study of metastasis is multifaceted and involves elements of such areas as differentiation, en zymology, genetics, hematology, immunology, membrane biochemistry and molecular biology. The major objectives of this book were to present the most recent advances in our understanding of how tumor cells metastasize to secondary sites by the leading experts in the biology of tumor invasion and metastasis. We hope that this book will lead to new concepts for the treatment of subclinical metastatic cancer. The chapters in this book address both the basic science of metastasis and potential clinical therapies directed toward interruption of the metastatic cascade or toward eradication of subclinical metastases. Many relevant topics have been omitted due to space considerations and thus the topics included reflect the prej udices of the editors.

Mammalian Cell Technology

Author : William G. Thilly
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Animal Cell Technology Basic Applied Aspects

Author : K. Nagai
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Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to uncover their abilities for industrial and medical purposes. The goal of animal cell technology includes clonal expansion of differentiated cells with useful abilities, optimization of their culture conditions on the industrial scale, modulation of their ability in order efficiently to produce medically and pharmaceutically important proteins, and application of animal cells to gene therapy and formation of artificial organs. This Volume gives the readers a complete review of the present state of the art in Japan, a country where this field is well advanced, as well as in Asia, Europe and the United States. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, biochemical engineers and those in other disciplines related to animal cell culture, working in academic environments as well as in the biotechnology and pharmaceutical industries.

Molecular and Cellular Biology of Multidrug Resistance in Tumor Cells

Author : I.B. Roninson
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The ability of neoplastic cells to survive exposure to various chemotherapeutic drugs represents the main obstacle to successful cancer chemotherapy. This book deals with a particular type of resistance in tumor cells that represents a single but especially important aspect of the multifaceted problem of cancer drug resistance. This type of resistance, known as multidrug or pleiotropic drug resistance, is characterized by cross-resistance of cells to several different classes of cytotoxic drugs, including some of the most commonly used anticancer agents. Over the last several years, there has been a veritable explosion of genetic, biochemical, and clinical information on multidrug resistance, which followed the identification and cloning of the genes responsible for this phenotype and the isolation of monoclonal antibodies against P-glycoproteins, the products of these genes. Elucida tion of the molecular mechanism of multidrug resistance has led to the formulation of novel approaches to the prediction of tumor response to chemotherapeutic drugs and increasing the efficacy of cancer therapy. Analysis of the structure and function of P glycoproteins from multidrug-resistant mammalian cells has also established a prototype for a novel class of eukaryotic membrane proteins, which have now been associated with a variety of transport processes in different organisms. This book summarizes the results of molecular biological, pharmacological, bio chemical, cytogenetic, immunological, and pathological studies on multidrug resistance in mammalian cells. Most of the chapters deal at least to some extent with the structure and expression of P-glycoprotein and its role in multidrug resistance.

Drug Resistance in Cancer Therapy

Author : Robert F. Ozols
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Where do you begin to look for a recent, authoritative article on the diagnosis or management of a particular malignancy? The few general oncology textbooks are generally out of date. Single papers in specialized journals are informative but seldom comprehensive; these are more often preliminary reports on a very limited number of patients. Certain general journals frequently publish good in-depth reviews of cancer topics, and published symposium lectures are often the best overviews available. Unfor tunately, these reviews and supplements appear sporadically, and the reader can never be sure when a topic of special interest will be covered. Cancer Treatment and Research is a series of authoritative volumes that aim to meet this need. It is an attempt to establish a critical mass of oncology literature covering virtually all oncology topics, revised frequently to keep the coverage up to date, easily available on a single library shelf or by a single personal subscription. We have approached the problem in the following fashion: first, by dividing the oncology literature into specific subdivisions such as lung can cer, genitourinary cancer, and pediatric oncology; second, by asking emi nent authorities in each of these areas to edit a volume on the specific topic on an annual or biannual basis. Each topic and tumor type is covered in a volume appearing frequently and predictably, discussing current diagnosis, staging, markers, all forms of treatment modalities, basic biology, and more.

International Journal of Radiation Biology

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Characterization of the Initial Products of Mammalian Gene Amplification and the Mechanisms of Their Formation

Author : Joseph C. Ruiz
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Genetics Abstracts

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The Genetic Basis of Human Cancer

Author : Bert Vogelstein
File Size : 27.31 MB
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Reveals what leading experts have recently discovered about cancers caused by DNA alterations! The second edition of THE GENETICS OF CANCER, newly titled THE GENETIC BASIS OF HUMAN CANCERS, updates and informs on the most recent progress in genetic cancer research and its impact on patient care. With contributions by the foremost authorities in the field, this fascinating new edition reports on how to understand and predict tumor development - information that can enhance decision-making and advance genetic research. 2ND Edition Highlights NEW CHAPTERS: * Peutz-Jeghers syndrome * Juvenile polyposis syndrome * Tumor genome instability * Gene expression profiling in cancer * Pilomatricoma and pilomatrix carcinoma * Hereditary paragangliomas of the head and neck * Cylindromatosis * Familial cardiac myxomas and carney complex * Cancers of the oral cavity and pharynx * Genetic abnormalities in lymphoid malignancies THOROUGHLY REVISED: * Every chapter has been meticulously reviewed and revised to incorporate the most recent research and clinical findings * Includes a valuable introduction by renowned editors Vogelstein & Kinser* Features 150 MORE illustrations than the previous edition

Cancer Research

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Induced Effects Of Genotoxic Agents In Eukaryotic Cells

Author : Toby G Rossman
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This book provides an understanding of the consequences of induced proteins in the toxicological response of cells to chemical and radiation damage to DNA and will be helpful in creating proper mathematical models for extrapolation to low doses and assessing human exposure or cellular injury.