Downstream Processing of Proteins

Methods and Protocols


Author: Mohamed A. Desai

Publisher: Springer Science & Business Media

ISBN: 1592590276

Category: Science

Page: 229

View: 2255

In Downstream Processing of Proteins: Methods and Protocols, Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods with a potential for scale-up. Also treated are the various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology. Every bioprocess scientist and engineer working to design and validate biological processes for novel proteins-and successfully apply for their new product licenses-will find this important book an eminently practical resource.


Issues in Environmental Ethics


Author: Donald Scherer

Publisher: Temple University Press

ISBN: 9780877227472

Category: Philosophy

Page: 242

View: 6621

Contains essays that explore non-reciprocated relationships with regard to the environment. This work includes contributions that discuss moral issues that arise when decisions by individuals, corporations, or governments cause changes in the environment that affect those who do not participate in the decisions.


Encounters with the Colorado River


Author: Karen Halverson

Publisher: N.A


Category: Travel

Page: 142

View: 9078

In both images and prose the book gives a view of the Colorado's great and enduring splendor, and a look at the many ironies contained in its waters."--BOOK JACKET.

Handbook of Downstream Processing


Author: E. Goldberg

Publisher: Springer Science & Business Media

ISBN: 9400915632

Category: Science

Page: 720

View: 8880

The last two decades have seen a phenomenal growth of the field of genetic or biochemical engineering and have witnessed the development and ultimately marketing of a variety of products-typically through the manipulation and growth of different types of microorganisms, followed by the recovery and purification of the associated products. The engineers and biotechnologists who are involved in the full-scale process design of such facilities must be familiar with the variety of unit operations and equipment and the applicable regulatory requirements. This book describes current commercial practice and will be useful to those engineers working in this field in the design, construction and operation of pharmaceutical and biotechnology plants. It will be of help to the chemical or pharmaceutical engineer who is developing a plant design and who faces issues such as: Should the process be batch or continuous or a combination of batch and continuous? How should the optimum process design be developed? Should one employ a new revolutionary separation which could be potentially difficult to validate or use accepted technology which involves less risk? Should the process be run with ingredients formulated from water for injection, deionized water, or even filtered tap water? Should any of the separations be run in cold rooms or in glycol jacketed lines to minimize microbial growth where sterilization is not possible? Should the process equipment and lines be designed to be sterilized in-place, cleaned-in-place, or should every piece be broken down, cleaned and autoclaved after every turn?

Downstream Industrial Biotechnology

Recovery and Purification


Author: Michael C. Flickinger

Publisher: John Wiley & Sons

ISBN: 111861898X

Category: Science

Page: 872

View: 7472

An affordable, easily accessible desk reference on biomanufacturing, focused on downstream recovery and purification Advances in the fundamental knowledge surrounding biotechnology, novel materials, and advanced engineering approaches continue to be translated into bioprocesses that bring new products to market at a significantly faster pace than most other industries. Industrial scale biotechnology and new manufacturing methods are revolutionizing medicine, environmental monitoring and remediation, consumer products, food production, agriculture, and forestry, and continue to be a major area of research. The downstream stage in industrial biotechnology refers to recovery, isolation, and purification of the microbial products from cell debris, processing medium and contaminating biomolecules from the upstream process into a finished product such as biopharmaceuticals and vaccines. Downstream process design has the greatest impact on overall biomanufacturing cost because not only does the biochemistry of different products ( e.g., peptides, proteins, hormones, antibiotics, and complex antigens) dictate different methods for the isolation and purification of these products, but contaminating byproducts can also reduce overall process yield, and may have serious consequences on clinical safety and efficacy. Therefore downstream separation scientists and engineers are continually seeking to eliminate, or combine, unit operations to minimize the number of process steps in order to maximize product recovery at a specified concentration and purity. Based on Wiley's Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, this volume features fifty articles that provide information on down- stream recovery of cells and protein capture; process development and facility design; equipment; PAT in downstream processes; downstream cGMP operations; and regulatory compliance. It covers: Cell wall disruption and lysis Cell recovery by centrifugation and filtration Large-scale protein chromatography Scale down of biopharmaceutical purification operations Lipopolysaccharide removal Porous media in biotechnology Equipment used in industrial protein purification Affinity chromatography Antibody purification, monoclonal and polyclonal Protein aggregation, precipitation and crystallization Freeze-drying of biopharmaceuticals Biopharmaceutical facility design and validation Pharmaceutical bioburden testing Regulatory requirements Ideal for graduate and advanced undergraduate courses on biomanufacturing, biochemical engineering, biophar- maceutical facility design, biochemistry, industrial microbiology, gene expression technology, and cell culture technology, Downstream Industrial Biotechnology is also a highly recommended resource for industry professionals and libraries.

Biotreatment, Downstream Processing and Modelling

With 32 Tables


Author: P. Bajpai,T. Scheper,P.K. Bajpai,B. Wang,P.L. Rogers,H.S. Shin

Publisher: Springer Science & Business Media

ISBN: 9783540614852

Category: Science

Page: 205

View: 520

This book provides an up-to-date and rapid introduction to an important and currently active topic in graph theory. The author leads the reader to the forefront of research in this area. Complete and easily readable proofs of all the main theorems, together with numerous examples, exercises and open problems are given. The book is suitable for use as a textbook or as seminar material for advanced undergraduate and graduate students. The references are comprehensive and so it will also be useful for researchers as a handbook.


Reflections on Brook Trout, Fly Fishing, and the Waters of Appalachia


Author: David L. O’Hara,Matthew T. Dickerson

Publisher: Wipf and Stock Publishers

ISBN: 1630873748

Category: Religion

Page: 146

View: 4753

Downstream: Reflections on Brook Trout, Fly Fishing, and the Waters of Appalachia is a mosaic combining nature writing, fly-fishing narrative, memoir, and philosophical and spiritual inquiry. Fly-fishing narratives and fragments of memoir provide the narrative arc for exploring relationships between humans and rivers, and the ways in which our attitudes and philosophies impact our practices and the waters we depend on for life. The authors guide their readers on a journey from Maine's Androscoggin watershed--once one of the ten filthiest rivers in the United States and now home to some of the best wild brook trout fishing in the United States--southward through Kentucky into Tennessee and North Carolina, where a native southern strain of brook trout struggles to survive. Like the rivers themselves, the chapters alternate between flowing narratives and the stiller waters that settle out above dams. While each stone in this mosaic is worth a close look in its own right, seen from a distance the book offers a broader picture of the cold mountain waters of Appalachia and their famous native fish: the brook trout.


Adaptive Management of Glen Canyon Dam and the Colorado River Ecosystem


Author: National Research Council,Division on Earth and Life Studies,Commission on Geosciences, Environment and Resources,Committee on Grand Canyon Monitoring and Research

Publisher: National Academies Press

ISBN: 0309184053

Category: Science

Page: 242

View: 3862

The Grand Canyon Monitoring and Research Center began long-term planning at its inception and, in May 1997, produced a Long-Term Monitoring and Research Strategic Plan that was adopted by stakeholder groups (the Adaptive Management Work Group and the Technical Work Group) later that year. The Center then requested the National Research Council's (NRC) Water Science and Technology Board to evaluate this plan.