High Resolution EPR

Applications to Metalloenzymes and Metals in Medicine

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Author: Graeme Hanson,Lawrence Berliner

Publisher: Springer Science & Business Media

ISBN: 0387848568

Category: Medical

Page: 666

View: 425

Metalloproteins comprise approximately 30% of all known proteins, and are involved in a variety of biologically important processes, including oxygen transport, biosynthesis, electron transfer, biodegradation, drug metabolism, proteolysis, and hydrolysis of amides and esters, environmental sulfur and nitrogen cycles, and disease mechanisms. EPR spectroscopy has an important role in not only the geometric structural characterization of the redox cofactors in metalloproteins but also their electronic structure, as this is crucial for their reactivity. The advent of x-ray crystallographic snapshots of the active site redox cofactors in metalloenzymes in conjunction with high-resolution EPR spectroscopy has provided detailed structural insights into their catalytic mechanisms. This volume was conceived in 2005 at the Rocky Mountain Conference on Analytical Chemistry (EPR Symposium) to highlight the importance of high-resolution EPR spectroscopy to the structural (geometric and electronic) characterization of redox active cofactors in metalloproteins. We have been fortunate to have enlisted internationally recognized experts in this joint venture to provide the scientific community with an overview of high-resolution EPR and its application to metals in biology. This volume, High-Resolution EPR: Applications to Metalloenzymes and Metals in Medicine, covers high-resolution EPR methods, iron proteins, nickel and copper enzymes, and metals in medicine. An eloquent synopsis of each chapter is provided by John Pilbrow in the Introduction. A second volume, Metals in Biology: Applications of High-Resolution EPR to Metalloenzymes, will appear later this year covering the complement of other metalloproteins. One of the pioneers in the development of pulsed EPR and its application to metalloproteins was Arthur Schweiger, whose contribution we include in this volume. Unfortunately, he passed away suddenly during the preparation of this volume. The editors and coauthors are extremely honored to dedicate this volume to the memory of Arthur Schweiger in recognition of his technical advances and insights into pulsed EPR and its application to metalloproteins. Arthur was extremely humble and treated everyone with equal respect. He was a gifted educator with an ability to explain complex phenomena in terms of simple intuitive pictures, had a delightful personality, and continues to be sadly missed by the community. It is an honor for the editors to facilitate the dissemination of these excellent contributions to the scientific community. Suggestions for future volumes are always appreciated.

Structure and Function

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Author: Peter Comba

Publisher: Springer Science & Business Media

ISBN: 9789048128884

Category: Science

Page: 260

View: 8563

The art of chemistry is to thoroughly understand the properties of molecular compounds and materials and to be able to prepare novel compounds with p- dicted and desirable properties. The basis for progress is to fully appreciate and fundamentally understand the intimate relation between structure and function. The thermodynamic properties (stability, selectivity, redox potential), reactivities (bond breaking and formation, catalysis, electron transfer) and electronic properties (spectroscopy, magnetism) depend on the structure of a compound. Nevertheless, the discovery of novel molecular compounds and materials with exciting prop- ties is often and to a large extent based on serendipity. For compounds with novel and exciting properties, a thorough analysis of experimental data – state-of-the-art spectroscopy, magnetism, thermodynamic properties and/or detailed mechanistic information – combined with sophisticated electronic structure calculations is p- formed to interpret the results and fully understand the structure, properties and their interrelation. From these analyses, new models and theories may emerge, and this has led to the development of ef cient models for the design and interpre- tion of new materials and important new experiments. The chapters in this book therefore describe various fundamental aspects of structures, dynamics and physics of molecules and materials. The approaches, data and models discussed include new theoretical developments, computational studies and experimental work from molecular chemistry to biology and materials science.

Advances in Carbohydrate Chemistry and Biochemistry

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Author: N.A

Publisher: Academic Press

ISBN: 0123855195

Category: Science

Page: 338

View: 2439

Since its inception in 1945, this serial has provided critical and integrating articles written by research specialists that bring together industrial, analytical and technological aspects of biochemistry, organic chemistry and instrumentation methodology in the study of carbohydrates. The articles provide a definitive interpretation of the current status and future trends in carbohydrate chemistry and biochemistry. Features contributions from leading authorities and industry experts Informs and updates on all the latest developments in the field

Microscopy Applied to Materials Sciences and Life Sciences

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Author: Ajay Vasudeo Rane,Sabu Thomas,Nandakumar Kalarikkal

Publisher: CRC Press

ISBN: 1351251570

Category: Science

Page: 502

View: 7399

This new volume, Microscopy Applied to Materials Sciences and Life Sciences. focuses on recent theoretical and practical advances in polymers and their blends, composites, and nanocompos¬ites related to their microscopic characterization. It highlights recent accomplishments and trends in the field of polymer nanocomposites and filled polymers related to microstructural characterization. This book gives an insight and better understanding into the development in microscopy as a tool for characterization. The book emphasizes recent research work in the field of microscopy in life sciences and materials sciences mainly related to its synthesis, characterizations, and applications. The book explains the application of microscopic techniques in life sciences and materials sciences, and their applications and state of current research carried out. The book aims to foster a better understanding of the properties of polymer composites by describing new techniques to measure microstructure property relationships and by utilizing techniques and expertise developed in the conventional filled polymer composites. Characterization techniques, particularly microstructural characterization, have proven to be extremely difficult because of the range of length-scales associated with these materials. Topics include: •Instrumentation and Techniques: advances in scanning probe microscopy, SEM, TEM, OM. 3D imaging and tomography, electron diffraction techniques and analytical microscopy, advances in sample preparation techniques in-situ microscopy, correlative microscopy in life and material sciences, low voltage electron microscopy. •Life Sciences: Structure and imaging of biomolecules, live cell imaging, neurobiology, organelles and cellular dynamics, multi-disciplinary approaches for medical and biological sciences, microscopic application in plants, microorganism and environmental science, super resolution microscopy in biological sciences. •Materials Sciences: materials for nanotechnology, metals alloys and inter-metallic, ceramics, composites, minerals and microscopy in cultural heritage, thin films, coatings, surfaces and interfaces, carbon based materials, polymers and soft materials and self-assembled materials, semiconductors and magnetic materials. Polymers and inorganic nanoparticles. The volume will be of significant interest to scientists working on the basic issues surrounding polymers, nanocomposites, and nanoparticulate-filled polymers, as well as those working in industry on applied problems, such as processing. Because of the multidisciplinary nature of this research, the book will be valuable to chemists, materials scientists, physicists, chemical engineers, and processing specialists who are involved and interested in the future frontiers of blends.

Metals in Biology

Applications of High-Resolution EPR to Metalloenzymes

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Author: Graeme Hanson,Lawrence Berliner

Publisher: Springer

ISBN: 1441911391

Category: Medical

Page: 419

View: 5881

Metal ions in biology is an ever expanding area in science and medicine involving metal ions in proteins and enzymes, their biosynthesis, catalysis, electron transfer, metal ion trafficking, gene regulation and disease. While X-ray crystallography has provided snapshots of the geometric structures of the active site redox cofactors in these proteins, the application of high resolution EPR spectroscopy in conjunction with quantum chemistry calculations has enabled, in many cases, a detailed understanding of a metalloenzymes mechanism through investigations of the geometric and electronic structure of the resting, enzyme-substrate intermediates and product complexes. This volume, Part II of a two-volume set demonstrates the application of high resolution EPR spectroscopy in determining the geometric and electronic structure of active site metal ion centers in iron sulfur cluster containing metalloproteins, mononuclear molybdenum metalloenzymes, manganese-containing enzymes and novel metalloproteins.

Magnetic resonance

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Author: P. T. Manoharan,Indian National Science Academy

Publisher: N.A

ISBN: N.A

Category: Medical

Page: 161

View: 2231

Resources for biomedical research technology

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Author: Research Resources Information Center,National Center for Research Resources (U.S.)

Publisher: N.A

ISBN: N.A

Category: Medical

Page: N.A

View: 7757

MRT-Atlas

Orthopädie und Neurochirurgie. Wirbelsäule

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Author: Martin Weyreuther,Christoph E. Heyde,Michael Westphal,Jan Zierski,Ulrich Weber

Publisher: Springer Science & Business Media

ISBN: 3540402853

Category: Medical

Page: 298

View: 2505

Das vorliegende Buch ist ein in interdisziplinärer Zusammenarbeit zwischen Radiologen, Orthopäden, Unfallchirurgen und Neurochirurgen erstellter klinisch orientierter MRT-Bildatlas. Wesentliche Erkrankungen und Verletzungen der Wirbelsäule werden dargestellt. Neben einem umfangreichen Bildmaterial werden Hinweise zur Anatomie und Pathophysiologie, zu den MRT-Zeichen physiologischer und pathologischer Befunde, zu diagnostischen Fallstricken und zur klinischen Wertigkeit der Befunde gegeben. Durch die gleichzeitige Darstellung von Klinik und MRT-Diagnostik soll das Einarbeiten in eine eigenständige Bildbeurteilung der Wirbelsäule erleichtert werden. Die Grundlagen sind übersichtlich und prägnant zum Verständnis von Anatomie und Pathologie präsentiert, Diagnostik und operative Maßnahmen werden diadaktisch geschickt aufbereitet.