Handbook of Isoelectric Focusing and Proteomics

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Author: David Edward Garfin,Satinder Ahuja

Publisher: Academic Press

ISBN: 9780120887521

Category: Medical

Page: 334

View: 9342

Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips * Is an ideal single source for students and researchers working in proteomics

Handbook of Bioseparations

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Author: Satinder Ahuja

Publisher: Elsevier

ISBN: 9780080507798

Category: Technology & Engineering

Page: 722

View: 3994

It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories. Key Features * Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies * Encompasses both analytical and process-scale methods * Elucidates the importance of engineering process control * Details selection of plant and process equipment * Addresses economic considerations * Discusses future developments

Low-Abundance Proteome Discovery

State of the Art and Protocols

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Author: Egisto Boschetti,Pier Giorgio Righetti

Publisher: Newnes

ISBN: 0124045960

Category: Science

Page: 364

View: 6598

Low-Abundance Proteome Discovery addresses the most critical challenge in biomarker discovery and progress: the identification of low-abundance proteins. The book describes an original strategy developed by the authors that permits the detection of protein species typically found in very low abundance and that may yield valuable clues to future discoveries. Known as combinatorial peptide ligand libraries, these new methodologies are one of the hottest topics related to the study of proteomics and have applications in medical diagnostics, food quality, and plant analysis. The book is written for university and industry scientists starting proteomic studies of complex matrices (e.g., biological fluids, biopsies, recalcitrant plant tissues, foodstuff, and beverage analysis), researchers doing wet chemistry, and graduate-level students in the areas of analytical and biochemistry, biology, and genetics. Covers methodologies for enhancing the visibility of low-abundance proteins which, until now, has been the biggest challenge in biomarker progress Includes detailed protocols that address real-life needs in laboratory practice Addresses all applications, including human disease, food and beverage safety, and the discovery of new proteins/peptides of importance in nutraceutics Compiles the research and analytic protocols of the two scientists who are credited with the discovery of these landmark methodologies, also known as combinatorial peptide ligand libraries, for the identification of low-abundance proteins

Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition

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Author: James P. Landers

Publisher: CRC Press

ISBN: 9781420004953

Category: Medical

Page: 1592

View: 4830

Although capillary electrophoresis (CE) technology has evolved quickly from the research laboratory into practical application in numerous fields, many scientists still debate its merits. While the body of international CE literature continues to expand dramatically, experts still question whether it has provided the speed, resolving power, peak capacity, sensitivity, robustness, and cost-reduction promised by its pioneers. Responding to these criticisms, this third edition brings together cutting-edge researchers to demonstrate the utility of CE across a broad spectrum of disciplines including— Forensic science Medical diagnostics Pharmaceutical science Genetic analysis Biotechnology Fluid mechanics Environmental science Biomedical research Nanotechnology Proteomics Detailed Analysis of New Methodologies and Applications Eagerly awaited by researchers and technicians who transformed the first two editions into bestsellers, this latest volume once again delivers. Emphasizing microseparations and microfluidics, the Handbook of Capillary and Microchip Electrophoresis, Third Edition features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Aided by contributions from leading international experts, this text remains a seminal reference for numerous chemistry, biology, and engineering fields.

Handbook of Detection of Enzymes on Electrophoretic Gels

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Author: Gennady P. Manchenko

Publisher: CRC Press

ISBN: 9781420040531

Category: Medical

Page: 568

View: 1514

Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first published, it remains the only book completely devoted to zymogram methods. The time has come to bring its contents up to date. New in the Second Edition: An overview of new applications of enzyme electrophoresis and zymogram techniques Zymogram techniques for approximately 100 enzymes not included in the first edition, bringing the total to more than 900 methods for detecting more than 400 different enzymes Information on subunit structure included within the enzyme sheets to facilitate interpretation of isozyme patterns detected on zymograms An appendix containing information on the buffer systems most commonly used for enzyme electrophoresis in starch, cellulose acetate, and polyacrylamide gels. The second edition of this bestselling handbook makes significant additions to the set of reliable gene markers suitable for electrophoretic analysis. It also strengthens the value of enzyme electrophoresis as a powerful tool proven successful in solving a variety of problems encountered across many biological areas, including the post-genome biology.

Henry's Clinical Diagnosis and Management by Laboratory Methods E-Book

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Author: Richard A. McPherson,Matthew R. Pincus

Publisher: Elsevier Health Sciences

ISBN: 1455726842

Category: Medical

Page: 1568

View: 4419

Recognized as the definitive book in laboratory medicine since 1908, Henry’s Clinical Diagnosis and Management by Laboratory Methods, edited by Richard A. McPherson, MD and Matthew R. Pincus, MD, PhD, is a comprehensive, multidisciplinary pathology reference that gives you state-of-the-art guidance on lab test selection and interpretation of results. Revisions throughout keep you current on the latest topics in the field, such as biochemical markers of bone metabolism, clinical enzymology, pharmacogenomics, and more! A user-friendly full-color layout puts all the latest, most essential knowledge at your fingertips. Update your understanding of the scientific foundation and clinical application of today's complete range of laboratory tests. Get optimal test results with guidance on error detection, correction, and prevention as well as cost-effective test selection. Reference the information you need quickly and easily thanks to a full-color layout, many new color illustrations and visual aids, and an organization by organ system. Master all the latest approaches in clinical laboratory medicine with new and updated coverage of: the chemical basis for analyte assays and common interferences; lipids and dyslipoproteinemia; markers in the blood for cardiac injury evaluation and related stroke disorders; coagulation testing for antiplatelet drugs such as aspirin and clopidogrel; biochemical markers of bone metabolism; clinical enzymology; hematology and transfusion medicine; medical microbiology; body fluid analysis; and many other rapidly evolving frontiers in the field. Effectively monitor the pace of drug clearing in patients undergoing pharmacogenomic treatments with a new chapter on this groundbreaking new area. Apply the latest best practices in clinical laboratory management with special chapters on organization, work flow, quality control, interpretation of results, informatics, financial management, and establishing a molecular diagnostics laboratory. Confidently prepare for the upcoming recertification exams for clinical pathologists set to begin in 2016.

Gentechnologie für Einsteiger

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Author: T. A. Brown

Publisher: Spektrum Akademischer Verlag

ISBN: 9783827428684

Category: Science

Page: 298

View: 4337

Gentechnologie für Einsteiger hat sich weltweit als leicht verständliche Einführung in dieses wichtige und spannende Wissenschaftsgebiet bewährt. Die sechste Auflage bleibt dem Grundkonzept früherer Auflagen treu, widmet sich aber auch neuen, wachsenden Forschungsfeldern. Das Buch bleibt damit ein unentbehrlicher Leitfaden für Studierende in den Biowissenschaften und ihren zahlreichen Teilgebieten. Es eignet sich auch bestens als Einführung für alle, die sich in ihrem Beruf mit den Grundlagen des Themas vertraut machen müssen.

Färbung von Lebensmitteln, Arzneimitteln, Kosmetika

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Author: Gisbert Otterstätter

Publisher: Behr's Verlag DE

ISBN: 3899479580

Category:

Page: 466

View: 334

Das Buch "Färbung von Lebensmitteln, Arzneimitteln, Kosmetika" wendet sich an Praktiker in der Lebensmittel-, Arzneimittel- und Kosmetikindustrie. Beschrieben werden anwendungstechnische Hinweise, einfache analytische Arbeitsmethoden sowie Informationen über die Zulässigkeit der Färbemittel in D, der EU und den wichtigsten Staaten außerhalb der EU. Von besonders großem Nutzen für die Praxis ist der sehr umfangreiche Block an Datenblättern der Färbemittel für Lebensmittel, Arzneimittel und Kosmetika! Die Datenblätter sind nach der Colour Indexnummer bzw. bei Färbemitteln in alphabetischer Reihenfolge geordnet.

Die Doppel-Helix

ein persönlicher Bericht über die Entdeckung der DNS-Struktur

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Author: James D. Watson

Publisher: N.A

ISBN: 9783499602559

Category: DNA

Page: 224

View: 9715

Technische Biochemie

Die Biochemie und industrielle Nutzung von Naturstoffen

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Author: Oliver Kayser,Nils Averesch

Publisher: Springer-Verlag

ISBN: 3658055480

Category: Science

Page: 242

View: 1991

Technik und Biologie muss kein Widerspruch sein. Auch Ingenieure müssen die Grundlagen der Biochemie in ihrem Studium lernen und verstehen. Dabei ist manches Standardlehrbuch häufig zu detailliert und umfassend. Die Autoren haben nach langen Gesprächen mit Studierenden und Dozenten den Versuch gewagt, ein Lehrbuch für Studierende der Bioingenieurwissenschaften auch aus dem Blickwinkel der Studierenden zu sehen und zu schreiben. Im Gegensatz zu bekannten und sehr gut etablierten Lehrbüchern der Biologie, Biochemie und Genetik, werden hier biologische Konzepte vorgestellt und mit technischen wie ingenieurwissenschaftlichen Problemen verknüpft. Das Anliegen dieses Lehrbuches ist, chemische und biochemische Prinzipien in der Naturstoffbiosynthese zu beleuchten und ihre biotechnischen und bioverfahrenstechnischen Herstellungswege zu erklären. Insbesondere die Darstellung im Text und auf Tafeln sollen so einen einfachen Zugang zu dem Netzwerk der Biochemie geben.

Pflanzenbiochemie

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Author: Hans Walter Heldt,Birgit Piechulla

Publisher: Springer-Verlag

ISBN: 3662443988

Category: Science

Page: 597

View: 7278

Die „Pflanzenbiochemie" hat sich im deutschsprachigen Raum, aber auch in zahlreichen Übersetzungen als Standardlehrbuch etabliert. Birgit Piechulla, Dozentin an der Universität Rostock, zeichnet als Co-Autorin bei dieser 5. Auflage verantwortlich und hat zusammen mit Hans-Walter Heldt das Buch gründlich überarbeitet und aktualisiert. Neueste wissenschaftliche Erkenntnisse fanden Eingang in dieses Buch, die sich auch in neuen Abbildungen sowie der stark überarbeiteten Literatur widerspiegeln. Besonderen Wert legen die Autoren darauf, die offenen, zukunftsweisenden Fragen, die den derzeitigen Stand unseres Wissens markieren, aufzuzeigen. Aktualität sowie die klare und verständliche Didaktik komplexer Sachverhalte darzustellen -- das sind die Kennzeichen dieses Lehrbuches. Mit sorgfältig erstellten zweifarbigen Abbildungen erfüllt es einen hohen didaktischen Anspruch und reiht sich unter die besten Biochemie-Lehrbücher.

Vademecum metabolicum

Diagnose und Therapie erblicher Stoffwechselkrankheiten

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Author: Johannes Zschocke,Georg Friedrich Hoffmann

Publisher: Schattauer Verlag

ISBN: 3794528158

Category:

Page: 174

View: 5052

Einführung in die Chromatographie

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Author: Roy J. Gritter,James M. Bobbitt,Arthur E. Schwarting

Publisher: Springer-Verlag

ISBN: 3642727891

Category: Science

Page: 274

View: 5999

Der Experimentator Molekularbiologie / Genomics

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Author: Cornel Mülhardt

Publisher: Springer-Verlag

ISBN: 3642346367

Category: Science

Page: 303

View: 7697

Dieses Buch enthält das Grundlagenwissen sowie Tipps und Tricks für den Umgang mit Nucleinsäuren. Der Autor kennt Lust und Frust der täglichen Laborroutine ganz genau. Präparieren, Fällen, Konzentrieren und Reinigen von Nucleinsäuren Restriktionsenzyme, Gele, Blotten Polymerase-Kettenreaktion RNA-Isolierung, -Transkription Klonierung von DNA-Fragmenten Markierung von Sonden, Hybridisierung, Screening, Sequenzierung Mutagenese, In-vitro-Translation, transgene Mäuse, Transgenexpression, Gentherapie, Genomik Dieses Buch richtet sich an alle Experimentatoren, die molekularbiologische Versuche durchführen wollen und gern nachvollziehen möchten, was sich in ihrem Reaktionsgefäß abspielt. Das ganze Spektrum der üblichen molekularbiologischen Methoden wird vorgestellt, kommentiert und Alternativen aufgezeigt. Der lockere Ton wendet sich gleichermaßen an Studenten wie an BTAs und Laboranten, aber auch der alte Hase wird hier und dort noch etwas Neues entdecken. Die 7. Auflage wurde überarbeitet und aktualisiert.

Mechanik

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Author: Charles Kittel

Publisher: Springer-Verlag

ISBN: 366314030X

Category: Science

Page: 316

View: 4583